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Surface-tension-induced budding drives alveologenesis in human mammary gland organoids

The development of glands involves cylindrical branches transforming into spherical alveoli. Now there is evidence to suggest that this process can be understood as a budding instability driven by a decrease in tension anisotropy in the tissue.

Organ development involves complex shape transformations driven by active mechanical stresses that sculpt the growing tissue(1,2). Epithelial gland morphogenesis is a prominent example where cylindrical branches transform into spherical alveoli during growth(3-5). Here we show that this shape transformation is induced by a local change from anisotropic to isotropic tension within the epithelial cell layer of developing human mammary gland organoids. By combining laser ablation with optical force inference and theoretical analysis, we demonstrate that circumferential tension increases at the expense of axial tension through a reorientation of cells that correlates with the onset of persistent collective rotation around the branch axis. This enables the tissue to locally control the onset of a generalized Rayleigh-Plateau instability, leading to spherical tissue buds(6). The interplay between cell motion, cell orientation and tissue tension is a generic principle that may turn out to drive shape transformations in other cell tissues.

https://www.webofscience.com/wos/woscc/full-record/WOS:000705937300027

Research Cooperation

CeNS stimulates and supports research cooperation - crossing the borders between disciplines and different institutions. Shared knowledge and know-how as well as continuous discussions between the members of the network promote synergy effects which boost scientific progress. CeNS continuously provides a multitude of different occasions for the researchers to meet and exchange ideas during scientific meetings and social events. Furthermore, the scientific board awards funding for cooperation projects of scientists belonging to different CeNS groups.

The successful collaborative research work between different CeNS scientists from Physics, Chemistry, Biology, Pharmacy and Medicine leads to joint research papers published in high-impact journals. The following list shows a selection of recently published cooperative papers:

Quantitative Single-Molecule Surface Enhanced Raman Scattering by Optothermal Tuning of DNA Origami-Assembled Plasmonic Nanoantennas

Sabrina Simoncelli, Eva-Maria Roller, Patrick Urban, Robert Schreiber, Andrew Turberfield, Tim Liedl, Theobald Lohmueller - ACS NANO, 10 (11):9809-9815; 10.1021/acsnano.6b05276 (2016)  more …

Theoretical and Experimental Study on the Optoelectronic Properties of Nb3O7(OH) and Nb2O5 Photoelectrodes

Khan, W., Betzler, S. B., Šipr, O., Ciston, J., Blaha, P., Scheu, C., Minar, J. - J. Phys. Chem. C, DOI: 10.1021/acs.jpcc.6b06391 (2016)  more …

Plasmon–Exciton Coupling Using DNA Templates

E. M. Roller, C. Argyropoulos, A. Hogele, T. Liedl and M. Pilo-Pais - Nano Letters, 10.1021/acs.nanolett.6b03015 (2016)  more …

Highly stable and biocompatible gold nanorod–DNA conjugates as NIR probes for ultrafast sequence-selective DNA melting

V. Baumann, P. J. F. Rottgermann, F. Haase, K. Szendrei, P. Dey, K. Lyons, R. Wyrwich, M. Grassel, J. Stehr, L. Ullerich, F. Bursgens and J. Rodriguez-Fernandez - Rsc Advances, 10.1039/c6ra17156g (2016)  more …

Conformational Changes and Flexibility of DNA Devices Observed by Small-Angle X-ray Scattering

L. K. Bruetzel, T. Gerling, S. M. Sedlak, P. U. Walker, W. J. Zheng, H. Dietz and J. Lipfert - Nano Letters, 10.1021/acs.nanolett.6b01338 (2016)  more …

Cell membrane penetration and mitochondrial targeting by platinum-decorated ceria nanoparticles

A. A. Torrano, R. Herrmann, C. Strobel, M. Rennhak, H. Engelke, A. Reller, I. Hilger, A. Wixforth and C. Bräuchle - Nanoscale, 10.1039/c5nr08419a (2016)  more …

Contractility as a global regulator of cellular morphology, velocity, and directionality in low-adhesive fibrillary micro-environments.

S. L. Schuster, F. J. Segerer, F. A. Gegenfurtner, K. Kick, C. Schreiber, M. Albert, A. M. Vollmar, J. O. Radler and S. Zahler - Biomaterials, 10.1016/j.biomaterials.2016.06.021 (2016)  more …

Tumoral gene silencing by receptor-targeted combinatorial siRNA polyplexes

D. J. Lee, D. S. He, E. Kessel, K. Padari, S. Kempter, U. Lachelt, J. O. Radler, M. Pooga and E. Wagner - Journal of Controlled Release, 10.1016/j.jconrel.2016.06.011 (2016)  more …

Dendronized mesoporous silica nanoparticles provide an internal endosomal escape mechanism for successful cytosolic drug release

V. Weiss, C. Argyo, A. A. Torrano, C. Strobel, S. A. Mackowiak, A. Schmidt, S. Datz, T. Gatzenmeier, I. Hilger, C. Bräuchle and T. Bein - Microporous and Mesoporous Materials, 10.1016/j.micromeso.2016.03.017 (2016)  more …

Ring-Shaped Microlanes and Chemical Barriers as a Platform for Probing Single-Cell Migration

C. Schreiber, F. J. Segerer, E. Wagner, A. Roidl and J. O. Radler - Scientific Reports, 10.1038/srep26858 (2016)  more …