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Tuesday, 23 February, 2010

Exploring the Conformation-Regulated Function of Titin Kinase by Mechanical Pump and Probe Experiments with Single Molecules

E. M. Puchner, H. E. Gaub -
Angew. Chem. int. Edition, Vol. 49 (6), Pages 1147 - 1150 (2010)

Feel the force: A new AFM-based single-molecule pump-and-probe protocol has been used to investigate the function of different force-induced conformations of proteins. It was found that the autoinhibited enzyme titin kinase reaches its ability to bind ATP after the first two barriers of the complex mechanical activation pathway.


Article on journal's website