Friday, 05 May, 2023

Directing Min protein patterns with advective bulk flow

S. Meindlhumer, F. Brauns, J. R. Finzgar, J. Kerssemakers, C. Dekker, E. Frey

DOI: 10.1038/s41467-023-35997-0

Meindlhumer et al. report a combined theoretical/experimental study of how the propagation direction of Min protein patterns can be altered by a bulk flow of solution.The Min proteins constitute the best-studied model system for pattern formation in cell biology. We theoretically predict and experimentally show that the propagation direction of in vitro Min protein patterns can be controlled by a hydrodynamic flow of the bulk solution. We find downstream propagation of Min wave patterns for low MinE:MinD concentration ratios, upstream propagation for large ratios, but multistability of both propagation directions in between. Whereas downstream propagation can be described by a minimal model that disregards MinE conformational switching, upstream propagation can be reproduced by a reduced switch model, where increased MinD bulk concentrations on the upstream side promote protein attachment. Our study demonstrates that a differential flow, where bulk flow advects protein concentrations in the bulk, but not on the surface, can control surface-pattern propagation. This suggests that flow can be used to probe molecular features and to constrain mathematical models for pattern-forming systems.