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CeNS Colloquium

Place: Kleiner Physik-Hörsaal, Geschwister-Scholl-Platz
Date: 23.07.10, Time: 15:30 h

Superresolution Fluorescence Microscopy and its Biological Application

Prof. Joerg Bewersdorf
Department of Cell Biology, Yale University School of Medicine, New Haven/CT

The power of fluorescence microscopy lies in the ability to directly observe sub-cellular structures, interactions and functions and to provide context for biochemical data.  It is, however, fundamentally limited by diffraction. Advances over the last decade have shown that the diffraction limit can be overcome enabling far-field microscopy at the sub-100 nm scale. This presentation will discuss some of the foundations of STED microscopy and Biplane photoactivation localization microscopy (Biplane FPALM) and their application in biological research. Biplane FPALM, a recent development in super-resolution 3D microscopy, generates images with approx. 25 x 25 x 75 nm resolution over a depth of several micrometers by combining a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) without compromising speed or sensitivity. Additionally, I will present data about a new fluorescent probe for STED microscopy.