Tuesday, 23 February, 2010
Exploring the Conformation-Regulated Function of Titin Kinase by Mechanical Pump and Probe Experiments with Single Molecules
E. M. Puchner, H. E. Gaub -
Angew. Chem. int. Edition, Vol. 49 (6), Pages 1147 - 1150 (2010)
Feel the force: A new AFM-based single-molecule pump-and-probe protocol has been used to investigate the function of different force-induced conformations of proteins. It was found that the autoinhibited enzyme titin kinase reaches its ability to bind ATP after the first two barriers of the complex mechanical activation pathway.