CeNS Colloquium
Date: 12.09.2022, Time: 14:00h
Location: Nano Institute, Königinstr. 57, Fakultät für Physik, LMU
Fluorescence nanoscopy with sub-10 nm resolution
Fernando D. Stefani
Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Ciudad Autónoma de Buenos Aires, Argentina
Super-resolution fluorescence microscopy, also known as fluorescence nanoscopy, represented a breakthrough for bioimaging as it delivers sub-diffraction resolution using far-field microscopes. Although they do not face any fundamental limit, the resolution of the first generation of methods was bound by the limited photostability of fluorophores under ambient conditions to about 10-30 nm resolution. This has motivated the development of a second generation of fluorescence nanoscopy methods that aim to surpass sub-10 nm resolution, thus providing true molecular resolution. In this talk, I will present latest efforts of our lab to address this challenge trough three different approaches: SIMPLER1, STED-FRET2, and RASTMIN3.
(1) Szalai et al. Three-Dimensional Total-Internal Reflection Fluorescence Nanoscopy with Nanometric Axial Resolution by Photometric Localization of Single Molecules. Nat. Commun. 2021, 12 (1), 517. doi.org/10.1038/s41467-020-20863-0.
(2) Szalai et al. Super-Resolution Imaging of Energy Transfer by Intensity-Based STED-FRET. Nano Lett. 2021, 21 (5), 2296-2303. doi.org/10.1021/acs.nanolett.1c00158.
(3) Masullo et al. An Alternative to MINFLUX That Enables Nanometer Resolution in a Confocal Microscope. Light Sci. Appl. 2022, 11 (1), 199. doi.org/10.1038/s41377-022-00896-4.