Wednesday, 19 September, 2012
Nanoscale Arrangement of Proteins by Single-Molecule Cut-and-Paste
M. Strackharn, D. Pippig, P. Meyer, S. Stahl, and H. Gaub -
J. Am. Chem. Soc., Vol. 134 (37), pp. 15193-15196 (2012)
Protein-based nanostructures are key to the organization of life and it is their precise arrangement, which determines their specific functions. A single-molecule approach for the directed assembly of protein arrangements allows for a controlled composition of systems based on protein components. Applying antibodies and antigenic peptide tags we utilized the Single-Molecule Cut-and-Paste (SMC&P) technique for the handling of single proteins. Protein–DNA complexes could be arranged to complex patterns with the functionality of the protein part remaining unimpaired.