Monday, 15 October, 2012
Label-Free Microscale Thermophoresis Discriminates Sites and Affinity of Protein–Ligand Binding
S. Seidel, C. Wienken, S. Geissler, M. Jerabek-Willemsen, S. Duhr, A. Reiter, D. Trauner, D. Braun, and P. Baaske -
Angew. Chem. Int. Edition, Vol. 51 (42), pp. 10656-10659 (2012)
The analysis of protein binding to small molecules, nucleic acids, and ions not only gives fundamental insights into cellular processes but also paves the way towards improved disease diagnosis and treatment. Herein, we report on a novel label- and preparation-free method to quantify biomolecular interactions and gather additional information on the binding event. The technique is based on the recently developed microscale thermophoresis (MST).