CeNS Center for NanoScience LMU Ludwig-Maximilians-Universität München
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Wednesday, 01 October, 2008

Nanoparticle Self-Assembly on a DNA-Scaffold Written by Single-Molecule Cut-and-Paste

E. M. Puchner, S. K. Kufer, M. Strackharn, S. W. Stahl, and H. E. Gaub -
Nano Lett., 8 (11), 3692

Self-assembly guided by molecular recognition has in the past been employed to assemble nanoparticle superstructures like hypercrystals or nanoparticle molecules. An alternative approach, the direct molecule-by-molecule assembly of nanoscale superstructures, was demonstrated recently. Here we present a hybrid approach where we first assemble a pattern of binding sites one-by-one at a surface and then allow different nanoparticles to attach by self-assembly. For this approach, biotin bearing DNA oligomers were picked up from a depot using a cDNA strand bound to an AFM tip. These units were deposited in the target area by hybridization, forming a recognition pattern on this surface. Fluorescent semiconductor nanoparticles conjugated with streptavidin were allowed to assemble on this scaffold and to form the final nanoparticle superstructures.


Article on journal's website