Wednesday, 02 June, 2010
Single-Molecule STED Microscopy with Photostable Organic Fluorophores
R. Kasper, B. Harke, C. Forthmann, P. Tinnefeld, S. W. Hell, and M. Sauer -
Small, Vol. 6, Issue 13, pp 1379 - 1384 (2010)
The combination of stimulated emission depletion (STED) microscopy with fluorophore stabilization through a reducing and oxidizing system (ROXS) enables the repetitive measurements necessary for 3D or dynamic STED imaging and resolution enhancement at the single-molecule level. A lateral resolution of <30 nm is obtained for raw image data recorded from single organic fluorophores immobilized in aqueous buffer.