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Pressemitteilung auf Deutsch

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LMU Press release... Go-Bio website]]>

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MinE conformational switching confers robustness on self-organized Min protein patterns

Jonas Denk*, Simon Kretschmer*, Jacob Halatek*, Caroline Hartl, Petra Schwille and Erwin Frey

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LMU Press release...]]>

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“When we walk across a bridge, this don’t affect the mechanical properties of this structure. Our study show that things are different for cells in tissues. As cells move through the matrix, they can pull on the structure to strongly enhance its stiffness”, explains Broedersz. This increase of matrix stiffness is made possible because the stiffness of biopolymer matrices is highly nonlinear: the matrix stiffens when it deforms, unlike most regular non-biological materials. Broedersz and coworkers exploited this nonlinearity to develop a new method to characterize cell-matrix interactions. Their so-called Nonlinear Stress Inference Microscopy (NSIM) makes it possible to infer mechanical stresses induced by the cell in 3D. By measuring these stresses with NSIM, they could understand how cells interact mechanically with their surrounding extracellular matrix. This study, published in PNAS highlights the importance of cellular stresses and matrix mechanics at the microscopic scale, and suggests a concrete mechanism through which cells can control their microenvironment and mechanically communicate with each other.

Using NSIM, Broedersz and colleagues could demonstrate that cell contraction induces large stresses, responsible for generating a massive stiffness gradient over an extended region in 3D matrices. “Interestingly, in all matrix model systems we investigated experimentally, we found a universal behavior: cell-induced stresses propagate over unexpectedly large ranges.”, says Broedersz, “Put simply, stresses created by the cell propagate as in a network of ropes. This is different from expectations based on elastic theories for ordinary materials. These active stresses generated by the cell are capable of exciting the matrix’s nonlinear stiffening over large distances.”

In their experimental study, 3D biological matrices with embedded cells were infused with latex beads. The biophysicists used optical tweezers to pull on these beads. This allowed them to measure the tension and stiffness of the matrix at different locations around the cell. “This enables us to directly measure how living cells mechanically modify their microenvironment.”, illustrates Broedersz. “The cell-induced stresses result in far-reaching stiffness changes. Other cells in the surrounding matrix could in principle sense and respond to these changes. This suggests that cell-induced matrix stiffening provides a concrete mechanism for mechanical communication between multiple cells in the matrix.” These observations emphasize the critical role of nonlinear matrix mechanics in shaping cell-matrix interactions, and may regulate cell behaviors and physiological functionalities. Thanks to the simplicity of the NSIM method, it could be used in various contexts, including embryo and tumor development. (NIM)

PNAS 2018 Source: Press release LMU Pressemitteilung auf Deutsch

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