PhD student in the group of Prof. Joachim Rädler, LMU Munich
2009 - 2010
Diploma Thesis in the group of Prof. Dieter Braun, LMU Munich
Topic of Diploma Thesis: "Thermophoretic Immunology"
2006 - 2010
Diploma in physics, LMU Munich
2007 - 2008
Erasmus scholarship, Universidad de Sevilla (Spain)
2004 - 2006
Studies in physics, TU Munich
Scholarship of the IDK-NBT (Elite Network of Bavaria)
The goal of my PhD thesis is to measure the length distribution and conformational change of recombinant GFP-VWF (Von Willebrand Factor) using fluorescence correlation spectroscopy (FCS).
FCS uses a confocal fluorescence setup to analyze the dynamics of macromolecules by detecting the intensity time trace of fluorescent molecules diffusing in and out of the small optical focus. It allows for measuring the diffusion constant, the detection of binding events, self-assembly and conformational changes in buffer as well as in complex media like blood plasma.
VWF exists as multimers with variable number of dimeric subunits. The protease ADAMTS 13 is responsible for the shape of the VWF length distribution and hence represents an important regulator of VWF function. We aim to establish a method that allows for monitoring the functionality of ADAMTS 13 in blood plasma.
Secondly we want to measure in the conformational changes of VWF under flow. A two-focus FCS will be developed as a fast flow-through setup to measure the degree of uncoiling of large multimers and to relate shear-induced changes to functionality such as binding events.
H. Engelke, S. Lippok, I. Dorn, R. R. Netz, and J. O. Rädler:
"FVIII Binding to PS Membranes Differs in the Activated and Non-Activated Form and Can Be Shielded by Annexin A5"
J. Phys. Chem. B, 2011, 115 (44), pp 12963-12970