HOMECONTACTSITE MAPIMPRINT
CeNS Center for NanoScience LMU Ludwig-Maximilians-Universität München
CeNS HomepageLMU Homepage
Home  >  Alumni

Sushi Madhira

Indian

Curriculum Vitae 

Since 2012

IDK Student Representative

Since 2010

PhD candidate in the group of Prof. Don Lamb, LMU Munich

2010

 Master’s Project at Nanotechnology Division of Indian Oil Corporation Ltd., R&D Centre, Faridabad, India 

Topic of Master Thesis: “Preparation of nanoadditives for corrosion inhibition in Residual fuel oil

2005 - 2010

Master of Science (Hons.) in Chemistry (Integrated Dual Degree) and Bachelor of Engineering (Hons.) in Computer Science , BITS-Pilani, K.K. Birla Goa Campus

 

Scholarships

Since 2010

Scholarship of the IDK-NBT (Elite Network of Bavaria)

Research Project

Biophysical processes at the cellular level are best understood when no ensemble average distorts the overall picture, but rather single molecules are investigated sequentially.

Foerster or Fluorescence resonance energy transfer (FRET) measured between a single donor fluorophore and a single acceptor fluorophore is an ideally suited technique to measure distances and dynamics at single-molecule level. Though single-pair FRET is a powerful tool to study biological molecules undergoing Intra- and Inter-molecular interactions, it is restricted to only one measurable distance per molecule of interest. Owing to this limitation, it cannot reveal intricate details of the global conformational changes during complex biomolecular interactions which are multi-dimensional. For large complexes, using multi-color FRET schemes helps reporting about conformational changes in separate domains of the macromolecule in real time and provides a more comprehensive picture.

In order to expand the single-molecule FRET to study complex molecular interactions, I have assembled a Four-Color Total Internal Reflection Fluorescence (TIRF) Microscope system which allows to measure distances between four fluorophores and thus gives insight into complex molecular dynamics. My research objective is to study DNA-Polymerase interactions and Dynamics of Protein Folding (Chaperones) by employing different multi-color FRET schemes.