PhD student in the group of Prof. Patrick Cramer, LMU Munich
2007 - 2009
Master of Science in biochemistry, LMU Munich
Topic of Master Thesis: " Crystal structure of the unique tandem SH2 domain of Candida glabrata Spt6 at 1.5Å resolution"
2006 - 2007
Studies in biochemistry, Freie-Universität Berlin
2001 – 2005
Bachelor in Biology, Beijing Normal University, China
Topic of Bachelor Thesis: "Screening and characterization of the lipase from the genomic DNA library of Bacillus psychrophilus"
Scholarship of the IDK-NBT (Elite Network of Bavaria)
The mRNA levels of genes in the cell are regulated both by transcription and RNA degradation. My project is focused on the study of the regulation on RNA degradation. In all tested organisms tested, RNA degradation is a prevalent activity. There are three major classes of intracellular RNA-degrading enzymes (ribonucleases or RNases). The RNases encoded by the genome often have overlapping activities, making redundancy a general feature of RNA degradation systems. With some important exceptions, mutation of a single RNA degradation enzyme does not generally result in a complete block to RNA degradation in either eukaryotes or bacteria. This redundancy presumably enhances the overall efficiency and robustness of degradation pathways.
In my research, I’ve optimized the adapted RNA labeling protocol of Dölken et. al. in yeast. And collaborate with the bioinformaticians in Achim Tresch group using the unpublished algorithm to calculate genome-wide the RNA half-lives, in wild type as well as in different mutants. Also, with an unpublished normalization method, we can now deal with the global RNA level dynamic changes. With the help of bioinformaticians, we are still trying to improve our method to get more precise RNA half-life.
B. Schwalb, D. Schulz, M. Sun, B. Zacher, S. Dümcke, D. E. Martin, P. Cramer P, A. Tresch:
„Measurement of genome-wide RNA synthesis and decay rates with Dynamic Transcriptome Analysis (DTA).“
Bioinformatics. 2012 Mar 15;28(6):884-5. Epub 2012 Jan 28. PubMed PMID: 22285829.
M. Sun, B. Schwalb, D. Schulz, N. Pirkl, S. Etzold, L. Larivière, K. C. Maier, M. Seizl, A. Tresch, P. Cramer:
„Comparative dynamic transcriptome analysis (cDTA) reveals mutual feedback between mRNA synthesis and degradation.“ Genome Res. 2012 Apr 16. [Epub ahead of print] PubMed PMID: 22466169.
M. Sun, L. Lariviere, S. Dengl, A. Mayer, P. Cramer:
"A tandem SH2 domain in transcription elongation factor Spt6 binds the phosphorylated RNA polymerase II CTD"
JBC Papers in Press, October 6, 2010, DOI 10.1074/jbc.M110.144568
S. Dengl, A. Mayer, M. Sun, P. Cramer:
"Structure and in vivo requirement of the yeast Spt6 SH2 domain"
Journal of molecular biology 389(1): 211-225 (2009)
C. Yi-Hung, M. Sun, P. Knochel:
"LiCl-Mediated Preparation of Functionalized Benzylic Indium(III) Halides and Highly Chemoselective Palladium-Catalyzed Cross-Coupling in a Protic Cosolvent"
Angewandte Chemie International Edition 48(12): 2236-2239 (2009)