Katrin Schneider

German

Maintaining the DNA methylation pattern over many cell divisions is crucial for proper cell function in mammals. Misregulation of this process is involved in cancer formation. The key protein in this process is the maintenance DNA methyltransferase 1 (Dnmt1), an essential epigenetic factor that reestablishes methylation of hemimethylated CpG sites generated during DNA replication in S phase.

Central goal of my PhD project is to elucidate the cell cycle dependent regulatory interactions modulating the function of Dnmt1 and associated factors and investigate how they act together in vivo in a mechanistic network. I will address this with complementing cutting-edge bioimaging techniques, including 3D super-resolution microscopy and fluorescence recovery after photobleaching (FRAP) in combination with novel kinetic modeling approaches. For the latter purpose I aim to develop integrated acquisition and evaluation tools for systematic higher-throughput analyses of such interaction networks.

G. Pichler, P. Wolf, C. S. Schmidt, D. Meilinger, K. Schneider, C. Frauer, K. Fellinger, A. Rottach and H. Leonhardt:
"Cooperative DNA and histone binding by Uhrf2 links the two major repressive epigenetic pathways"
J Cell Biochem 112, 2585-93 (2011)

M. C. Cardoso, K. Schneider, R. M. Martin and H. Leonhardt:
"Structure, function and dynamics of nuclear subcompartments"
Curr Opin Cell Biol 24, 79-85 (2012)

T. Lebold, A. Schlossbauer, K. Schneider, L. Schermelleh, H. Leonhardt, T. Bein and C.Bräuchle:
"Controlling The Mobility Of Oligonucleotides In The Nanochannels Of Mesoporous Silica"
Advanced Functional Materials 22, 106-112 (2012)

C. Bönisch, K. Schneider, S. Pünzeler, S. M. Wiedemann, C. Bielmeier, M. Bocola, H. C. Eberl, W. Kuegel, J. Neumann, E. Kremmer, H. Leonhardt, M. Mann, J. Michaelis, L. Schermelleh and S. B. Hake:
"H2A.Z.2.2 is an alternatively spliced histone H2A.Z variant that causes severe nucleosome destabilization"
Nucleic Acids Research (2012)