2009 - 2010
IDK Student Representative
PhD student in the group of Prof. Christoph Bräuchle, LMU Munich
Master thesis in the group of Prof. Christoph Bräuchle, LMU Munich
Master Thesis: functionalization of mesoporous systems
2005 - 2007
Master of science in chemistry at the LMU Munich
2006 - 2006
Internship at the research group of Prof. P. Lincoln, Chalmers. University of Technology, Gothenburg, Sweden.
Research project: Intercalation of Ru(II) Complexes in DNA
Scholarship of the IDK-NBT (Elitenetwork of Bavaria)
Due to a rising amount of nanoparticles (NPs) in the environment leading to a constant exposure of the human body, NP uptake via our airways system, gastro-intestinal tract and skin is unavoidable. As NPs are able to penetrate physiological barriers they enter our blood and lymphatic vessel system which is covered by endothelial cells (ECs). These cells sense all intravascular physiological and artificial components. Moreover they play an essential role in controlling inflammation, coagulation, blood flow and blood pressure. Hence it is crucial to know the precise effect of NPs on these cells.
In my PhD project the effect of various kinds of industrial relevant NPs (e.g. Au, Ag, Pt) on living ECs will be studied by fluorescence microscopy and single NP tracking. These techniques allow to monitor the uptake of single NPs in living cells and to investigate the interaction with specific cell components. To mimic in vivo conditions this technique will be combined with a microfluidic system allowing to establish a blood flow like microfluidic flow on top of the ECs. Moreover the bioactivity of the ECs will be studied simultaneously by sophisticated essays. The project is funded by the DFG priority program (SPP 1313) “Bio-Nano-Responses”. The experiments will be done in collaboration with the groups of Prof. A. Reller (synthesis and characterization of nanoparticles), Prof. A. Wixforth and Dr. M. F. Schneider (Lab on a Chip systems, micro and nanofluidics) and PD Dr. S. W. Schneider (endothelial cell biology).
J. Blechinger, R. Herrmann, D. Kiener, F. J. García-García, C. Scheu, A. Reller, C. Bräuchle:
"Perylene-Labeled Silica Nanoparticles: Synthesis and Characterization of Three Novel
Silica Nanoparticle Species for Live-Cell Imaging"
first published online: 27 SEP 2010 small 2010
T. Lebold, L.A. Mühlstein, J. Blechinger, M. Riederer, H. Amenitsch, R. Köhn, K. Peneva, K. Müllen, J. Michaelis, C. Bräuchle, and T. Bein:
"Tuning single-molecule dynamics in functionalized mesoporous silica"
Chem. Eur. J. 15, 1661-167 (2009)
F. Westerlund, P. Nordell, J. Blechinger, T.M. Santos, B. Norden, and P. Lincoln:
"Complex DNA binding kinetics resolved-by combined circular dichroism and luminescence analysis"
Journal of Physical Chemistry B, 112(21): p. 6688-6694 (2008)