Ingo Stein
German
Curriculum Vitae
2011 | IDK Student Representative |
Since 2009 | PhD student in the group of |
2008 – 2009 | Diploma Thesis in the group of Prof. Philip Tinnefeld, LMU Munich |
Topic of Diploma Thesis: “Steroid Binding to a DNA Three-Way-Junction Visualized by Multicolor Single-Molecule Spectroscopy” | |
2006 | Studies at the Department of Physics at the University of Colorado at Boulder |
2003 – 2009 | Diploma in Physics at the LMU Munich |
Scholarships
Since 2009 | Scholarship of the IDK-NBT (Elite Network of Bavaria) |
Since 2004 | e-fellows.net scholarship |
2006 | DAAD scholarship for an exchange semester at the University of Colorado at Boulder |
Research Project
My area of interest is focused on the development and application of advanced fluorescence microscopy techniques, especially for multicolor excitation and detection of single molecules. For example, the concept of alternating laser excitation (ALEX) can be used to investigate the structure and stoichiometry of dye-labeled molecules, by using FRET (Fluorescence Resonance Energy Transfer) as a “nanoscopic ruler”.
Employing the DNA origami technique, introduced by Paul Rothemund in 2006, to build structures on the nanometer scale by DNA self-assembly can be directly applied to single-molecule fluorescence spectroscopy. A rigid DNA origami block was designed as a calibration ruler for single-molecule FRET measurements. In another project a DNA origami rectangle was used as a breadboard on which alternative FRET pathways could be visualized by a 4-color FRET approach witch alternating laser excitation (see figure).
Additionally I am interested in understanding the intrinsic properties of fluorescent molecules, which can be seen as a crucial prerequisite for any fluorescence microscopy technique. For instance the understanding and control of the blinking behavior of fluorescent dyes has shown to be beneficial for novel approaches to super-resolution imaging, e.g. “Blink Microscopy”.
- A DNA origami rectangle is used as breadboard for a spectroscopic network: the alternative FRET pathways are mediated by a green “jumper” dye that guides the light from the blue input dye to either the red or IR output. (Stein et al. JACS 2011)
Publications
G. P. Acuna, M. Bucher, I. H. Stein, C. Steinhauer,A. Kuzyk, P.Holzmeister, R. Schreiber, A. Moroz, F. D. Stefani, T. Liedl, F. C. Simmel, and P. Tinnefeld:
"Distance Dependence of Single-Fluorophore Quenching by Gold Nanoparticles Studied on DNA Origami"
ACS Nano, published online March 22, 2012
I.H. Stein, S. Capone, J.H. Smit, F. Baumann, T. Cordes, and P. Tinnefeld:
"Linking Single-Molecule Blinking to Chromophore Structure and Redox Potentials"ChemPhysChem, 13(4), 931-937, (incl. cover feature) (2012)
Article first published online: 8 Decemeber 2011
I.H. Stein, C. Steinhauer, and P. Tinnefeld:
"Single-Molecule Four-Color FRET Visualizes Energy-Transfer Paths on DNAOrigami"JACS, 133 (12), (incl. Cover Figure) (2011)
I.H. Stein*, V. Schüller*, P. Böhm, P. Tinnefeld, and Tim Liedl:
"Single-molecule FRET ruler based on rigid DNA origami blocks"ChemPhysChem 12(3), 689 - 695 *equal contribution (2011)
Article first published online: 9 FEB 2011
Ingo H. Stein, Philip Tinnefeld:
"Single-Molecule FRET & Super-Resolution: DNA Nanostructures Organize Dyes for Nanophotonic Apps"GIT Imaging and Microscopy 3, 2011
J. Vogelsang, C. Steinhauer, C. Forthman, I. H. Stein, B. Person, T. Cordes,P. Tinnefeld:
"Make them Blink: Probes for Super-Resolution Microscopy"
ChemPhysChem 11(12), 2475-2490 (2010)
B. Person, I. H. Stein, C. Steinhauer, J. Vogelsang and P. Tinnefeld:
"Correlated movement and bending of nucleic acid structures visualized by multicolor single-molecule spectroscopy"ChemPhysChem, 10(9-10):1455-1460, (2009)
T. Cordes, I. H. Stein, C. Forthmann, C. Steinhauer, M. Walz, W. Summerer, B. Person, J. Vogelsang and P. Tinnefeld:
"Controlling the emission of organic dyes for high sensitivity and superresolution microscopy"Proc SPIE, 7367:73671D, (2009)
ingo.stein
physik.uni-muenchen.de