Ingo Stein

German

Curriculum Vitae

2011	IDK Student Representative
Since 2009	PhD student in the group of Prof. Philip Tinnefeld, now TU Braunschweig
2008 – 2009	Diploma Thesis in the group of Prof. Philip Tinnefeld, LMU Munich
	Topic of Diploma Thesis: “Steroid Binding to a DNA Three-Way-Junction Visualized by Multicolor Single-Molecule Spectroscopy”
2006	Studies at the Department of Physics at the University of Colorado at Boulder
2003 – 2009	Diploma in Physics at the LMU Munich

Scholarships

Since 2009	Scholarship of the IDK-NBT (Elite Network of Bavaria)
Since 2004	e-fellows.net scholarship
2006	DAAD scholarship for an exchange semester at the University of Colorado at Boulder

Research Project

Single-Molecule Fluorescence Spectroscopy Measurements on the level of single molecules have become a routine practice in many laboratories and have started contributing to unravel fundamental biological questions. In general, single molecule techniques offer a number of distinct advantages over ensemble measurements. They can provide information on distributions and time trajectories of observables that would otherwise be obscured by the ensemble. Especially, individual species of a heterogeneous population can be individually identified and quantitatively observed for relevant properties. Therefore, to follow time trajectories of dynamics or for unravelling structural changes in individual populations, single molecule methods are most suited. My area of interest is focused on the development and application of advanced fluorescence microscopy techniques, especially for multicolor excitation and detection of single molecules. For example, the concept of alternating laser excitation can be used to investigate the structure and stoichiometry of dye-labeled molecules, by employing FRET (Fluorescence Resonance Energy Transfer) as a “nanoscopic ruler”.

Additionally I am interested in understanding the intrinsic properties of fluorescent molecules, which can be seen as a crucial prerequisite for any fluorescence microscopy technique. For instance the understanding and control of the blinking behavior of fluorescent dyes has shown to be beneficial for novel approaches to super-resolution imaging, e.g. “Blink Microscopy”.

Publications

I.H. Stein, S. Capone, J.H. Smit, F. Baumann, T. Cordes, and P. Tinnefeld:
Linking Single-Molecule Blinking to Chromophore Structure and Redox Potentials.
ChemPhysChem, published online December 8, 2011

I.H. Stein, C. Steinhauer, and P. Tinnefeld:
"Single-Molecule Four-Color FRET Visualizes Energy-Transfer Paths on DNAOrigami"
JACS, 133 (12), (incl. Cover Figure) (2011)

I.H. Stein*, V. Schüller*, P. Böhm, P. Tinnefeld, and Tim Liedl:
"Single-molecule FRET ruler based on rigid DNA origami blocks"
ChemPhysChem 2011, 12, 689 – 695 *equal contribution
Article first published online: 9 FEB 2011

J. Vogelsang, C. Steinhauer, C. Forthman, I. H. Stein, B. Person, T. Cordes,P. Tinnefeld:
"Make them Blink: Probes for Super-Resolution Microscopy"
ChemPhysChem 11:2475-2490 (2010)

B. Person, I. H. Stein, C. Steinhauer, J. Vogelsang and P. Tinnefeld:
"Correlated movement and bending of nucleic acid structures visualized by multicolor single-molecule spectroscopy"
ChemPhysChem, 10(9-10):1455-1460, (2009)

T. Cordes, I. H. Stein, C. Forthmann, C. Steinhauer, M. Walz, W. Summerer, B. Person, J. Vogelsang and P. Tinnefeld:
"Controlling the emission of organic dyes for high sensitivity and superresolution microscopy"
Proc SPIE, 7367:73671D, (2009)

ingo.steinphysik.uni-muenchen.de