Elisângela Moura Linares
PhD student in the group of PD Dr. Stephan Thalhammer, HelmholtzZentrum München and LMU Munich
2007 - 2009
Master of Science in Physical Chemistry, University of Campinas, Brazil
Master thesis: "Microstructural characterization of latex blend films"
2007 - 2008
Bachelor of Science in Technological Chemistry, University of Campinas, Brazil
2003 - 2006
Bachelor of Science in Chemistry, University of Campinas, Brazil
Scholarship of the IDK-NBT (Elitenetwork of Bavaria)
2007 - 2009
Fellowship from São Paulo Research Foundation (FAPESP)
2004 - 2006
Fellowship from São Paulo Research Foundation (FAPESP)-undergraduate student
Development of a microfluidic diagnostic detection device
In emerging and developing countries, there is a necessity for new health technology development for diagnosing diseases that allow assessment of individuals at point-of-care. Based on this challenge, my project aims to develop cheap devices which detect human pathogens or track environmental related pathogens affecting developing countries. These devices are based on lateral flow immunoassay (LFIA) which follows the same principles of ELISA test. LFIA consists of multiple layer membranes with pre-loaded specific antibody and it is actuated by capillary forces. The detection system is based on real-color changes and can determine analyte at very low concentrations exploring nanotechnology tools. The advantage of this device is that it is simple, fast to use and low cost when manufactured in large scale. It is capable of being performed by unskilled operators and it provides rapid and reliable results when applied to a wide variety of point-of-care or field use applications, requiring no instrumentation or extrinsic reading assistance.
E. M. Linares, C. S. Pannuti, L. T. Kubota, S. Thalhammer:
"Immunospot assay based on fluorescent nanoparticles for Dengue fever detection"
Biosensors and Bioelectronics 2012, accepted
E. M. Linares, L. T. Kubota, J. Michaelis, S. Thalhammer:
"Enhancement of the detection limit for lateral flow immunoassays: evaluation and comparison of bioconjugates."
Journal of Immunological Methods 2011, DOI: 10.1016/j.jim.2011.11.003
E. M. Linares, S. A. V. Jannuzzi, F. Galembeck:
"Electrostatic contributions in the increased compatibility of polymer blends."
Langmuir 2011, DOI: 10.1021/la2029998
M. M. Rippel, E. M. Linares, F. C. Bragança, L. F. Valadares, F. Galembeck:
"Electrostatic adhesion: an effective mechanism for rubber adhesion and blending."
Journal of Adhesion Science and Technology, 2011, In press.
E. M. Linares, M. M. Rippel, F. Galembeck:
"Clay platelet partition within polymer latex blend films: an EFTEM study"
ACS Appl. Mater. Interfaces, 2 (12), pp 3648–3653 (2010)
E. M. Linares, L. F. Valadares, C. A. Silva, C. A. Rezende, F. Galembeck:
"Molecular mapping by low-loss-energy EFTEM imaging"
Analytical Chemistry 81, 2317 (2009)
L. F. Valadares, E. M. Linares, F. C. Braganca, F. Galembeck:
"Electrostatic adhesion of nanosized particles: the cohesive role of water"
Journal of Physical Chemistry C, 112, 8534 (2008)
E. M. Linares, L. P. Trombeta, A. B. Moreira, P. Sotomayor, L. T. Kubota:
"A fluorescence spot test for salicylate determination"
Analytical Letters, 40, 573. (2007)