Prof. Patrick Cramer, Gene Center, Ludwig-Maximilians-University Munich (LMU)

Project:
The structure and function of a gene promoter, the region of DNA before the gene, is not well understood, although it is central for the regulation of gene expression in all living cells. We have established a system that enables us to measure the strength of a gene promoter both in vitro and in vivo. We have also conducted a bioinformatic analysis and identified potential new promoter elements. The new PhD student will use the established system and predictions to test which of the new elements is part of a functional gene promoter. This work will lead to a better understanding of transcription initiation from promoters and gene control. It is possible to extend this work together with other people in the lab to arrive at a more systemic, genomic view of promoter structure and function.

Techniques:
DNA cloning, transcription assays, in vivo reporter gene assays, microarrays, computational biology

Required skills:
Basic knowledge of biochemistry, molecular biology, and computing.

More information:
www.LMB.uni-muenchen.de/cramer