PhD Position – Super Resolution Microscopy of Virus-Cell Interactions
Project: Traditionally, optical microscopy is limited by diffraction to a resolution of approximately half the wavelength of light or, for visible light, ~ 200 nm. With current technology, it is now possible circumvent this limited and obtain optical resolution in all three dimensions of better than 100 nm. This is a range that becomes of high interest when investigating cellular processes.
In my group, we develop advanced fluorescence technologies and apply them to interesting biological problems. In the area of super resolution spectroscopy, we have two home-built instruments, one that is capable of performing STochastic Optical Reconstruction Microscopy (STORM, Rust, M. J.; Bates, M.; Zhuang, X. Nat Methods 2006, 3, 793) and a second capable of performing STimulated Emission Depletion Spectroscopy (STED, ). One of the goals of our research is to elucidate the lifecycle of Viruses such as HIV and Foamy Virus. Super resolution Spectroscopy gives us the opportunity to investigate the structure and protein complexes that interaction with viruses during the process of assembly and/or cell entry. The goal of this PhD project is to establish three-dimensional STED and STORM in our laboratory and use three-dimensional super resolution microscopy to investigate the structure of different virus-protein complexes at different stages in the viral lifecycle.
Within my group, we offer the PhD student an interdisciplinary work environment with graduate students and postdoctoral researchers expertise in physics, chemistry, biochemistry and biology. My laboratory is well equipped with 11 different advanced fluorescence microscopes.
Requirements: Candidates must be highly motivated, hard working and interested in interdisciplinary research, optical microscopy, quantitative analysis and biology. A degree in physics, physical chemistry, engineering, biochemistry or a related subject is recommended. Experience in optics, fluorescence microscopy and/or cell biology is beneficial but not necessary.
Recent related publications from my group:
Ivanchenko, S.; Godinez, W. J.; Lampe, M.; Kräusslich, H.-G.; Eils, R.; Rohr, K.; Bräuchle, C.; Müller, B.; Lamb, D. C. PLoS Pathogens 2009, 5, e1000652.
Baumgärtel, V.; Ivanchenko, S.; Dupont, A.; Sergeev, M.; Wiseman, P. W.; Kräusslich, H.-G.; Bräuchle, C.; Müller, B.; Lamb, D. C. Nature Cell Biology 2011, 13, 469.
Baumgartel, V.; Muller, B.; Lamb, D. C. Viruses 2012, 4, 777.
Stirnnagel, K.; Schupp, D.; Dupont, A.; Kudryavtsev, V.; Reh, J.; Mullers, E.; Lamb, D. C.; Lindemann, D. Retrovirology 2012, 9, 71.
Dupont, A.; Stirnagel, K.; Lindemann, D.; Lamb, D. C. Biophys. J. 2013, 104, 2373.
Group: Prof. Don Lamb, Department of Chemistry, LMU Munich
Prof. Lamb's website