Place: Adolf-von-Baeyer-Hörsaal, Butenandtstr. 5-13
Date: 17.12.10, Time: 15:30 h
Single-molecule studies of multi-protein machines
Prof. Antoine van Oijen
Zernike Institute for Advanced Materials, Centre for Synthetic Biology, University of Groningen
Novel nanomanipulation and imaging methods have made it possible to study biochemical reactions at the level of individual proteins. In a biological context, most of these proteins function in concert with others in multi-protein complexes, so an important future direction is the utilization of single-molecule techniques to unravel the orchestration of large macromolecular assemblies.
I will discuss our single-molecule studies of the replisome, the multi-protein machinery that is responsible for replication of DNA. I will present experiments that rely on the readout of mechanical DNA properties as well as single-molecule fluorescence imaging to obtain information on the catalytic activity and dynamic composition of the replisome. Further, I will present recent work on the visualization of the mechanisms that membrane-enveloped viruses, such as influenza and HIV, use to fuse their lipid membrane to that surrounding the cell.